Determination of Main Components in Idrocilamide Ointment by HPLC
- VernacularTitle:HPLC法测定羟乙桂胺乳膏中主药的含量
- Author:
Weijuan ZHAO
;
Yan CHEN
- Publication Type:Journal Article
- Keywords:
Idrocilamide;
Ointment;
HPLC;
Content determination
- From:
China Pharmacy
2007;0(25):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method to determine the content of main component in idrocilamide ointment. METHODS: The samples were separated on HP-ODS column with column temperature maintained at room temperature. The mobile phase consisted of methanol-water (65∶35) with a flow rate of 1.0 mL?min-1. The detection wavelength was set at 276 nm and the injection volume was 20 ?L. RESULTS: The linear range of idrocilamide was 0.05～4 ?g?mL-1,the average recovery rate was 99.97% (RSD=0.80%). CONCLUSION: This method is sensitive,accurate and applicable for the determination of the content of main component in idrocilamide ointment.
