Optimization of the Extraction Technology of Ardicrenin from Ardisia crenata by Orthogonal Experiment
- VernacularTitle:正交试验优选朱砂根中朱砂根皂苷提取工艺
- Author:
Hua ZHEN
;
Yijun FAN
;
Xueyi ZHANG
- Publication Type:Journal Article
- Keywords:
Ardisia crenata;
Ardicrenin;
RP-HPLC;
Orthogonal experiment;
Extraction technology
- From:
China Pharmacy
2005;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To optimize the extraction technology of ardicrenin from Ardisia crenata.METHODS: The content of ardicrenin was determined by RP-HPLC. The extraction technology was optimized by orthogonal experiment with the extraction rate of ardicrenin as evaluation index and with the volume fraction of ethanol,the extraction temperature,extraction time and extraction times as factors.RESULTS: The optimum extraction technology was determined as follows: 80%ethanol was used as solvent;the extraction temperature was 80 ℃;the extraction time was 20 min and the extraction was conducted for 3 times.The extraction rate of ardicrenin was 6.04%.CONCLUSION: The optimized extraction technology is feasible and reproducible,and it provides theoretical basis for mass extraction of the ardicrenin from A.crenata.
