Determination of Astragaloside Ⅳ in Xin'an Granules by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定心安颗粒中黄芪甲苷的含量
- Author:
Kailian ZHANG
;
Sijin YANG
- Publication Type:Journal Article
- Keywords:
Xin'an granules;
Astragaloside Ⅳ;
HPLC;
ELSD;
Content determination
- From:
China Pharmacy
2005;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: A HPLC-ELSD method was developed for determination of the content of astragaloside Ⅳ in Xin'an granules.METHODS: The separation was performed on Kromasil ODS C18 column(150 mm?4.6 mm,5 ?m) with the mobile phase consisted of acetonitrile-water(35∶65).The temperature of the drift tube was 40 ℃ and the flow rate of gas(air) was 2.1 L?min-1.RESULTS: The linear range of astragaloside Ⅳ was 1.32～7.95 ?g(r=0.998 9) and the average recovery was 97.84(RSD=1.01%,n=9).CONCLUSION: The method is simple,accurate and reproducible,and it can be used for the quality control of Xin'an granules.
