Apoptosis Expression-based Pattern Recognition System for Four Natures of Chinese Materia Medica——Comparative Study on Drug Natures of the Water Extracts of Panax ginseng,Dry Panax ginseng and Panax quinque folium against A?_(25-35)-induced Apoptosis of
- VernacularTitle:基于细胞凋亡表达的中药四性模式识别系统研究——红参、生晒参和西洋参抗A?_(25-35)诱导SH-SY5Y细胞凋亡的药性学比较
- Author:
Huimin YU
- Publication Type:Journal Article
- Keywords:
SH-SY5Y cells;
?-amyloid protein;
Drug nature;
Panax ginseng;
Dry Panax ginseng;
Panax quinque folium;
Water extract;
Apoptosis
- From:
China Pharmacy
2005;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of water extracts of Panax ginseng(warm),dry P.ginseng(mild),P.quinque folium(cool) on the apoptosis of SH-SY5Y cells induced by ?-amyloid protein(A?25-35).METHODS:SH-SY5Y cells were treated with A?25-35 to simulate the pathologic neuronic damage model in patients with Alzheimer's disease,and then treated with different doses of water extracts of P.ginseng(WERG),dry P.ginseng(WEG)or P.quinque folium(WEAG).The morphologic changes of the SH-SY5Y cells were observed by inverted microscope.Cell survival rate was measured by MTT assay.The percentage of apoptosis and the expression of Bax and Bcl-2 proteins were detected by Western Blotting.RESULTS: After treatment with A?25-35 50 ?mol?L-1 for 72 hours,SH-SY5Y cells became more round and aggregated and the cell survival rate was significantly reduced(P
