Content Determinaton of Main Component in Low Concentration of Alprostadil for Injection by HPLC
- VernacularTitle:HPLC法测定低浓度注射用前列地尔中主药的含量
- Author:
Lin LI
- Publication Type:Journal Article
- Keywords:
Low concentration;
Alprostadil for injection;
HPLC;
Diode array detector;
Content determination
- From:
China Pharmacy
2007;0(25):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an HPLC method for determination of main component in low concentration of alprostadil for injection. METHODS: Diode array detector was used for full wavelength scanning of the low concentration of alprostadil sample solution (20 ?g?mL-1) with detection wavelength set at 214 nm and the reference wavelength at 250 nm. The mobile phase consisted of acetonitrile-0.02 mol?L-1 KH2PO4 solution (pH 4.9,40∶60) at a flow rate of 1 mL?min-1. RESULTS: The linear range of alprostadil was 10～320 ?g?mL-1 (r=0.999 9) and the average recovery rate was 98.26% (RSD=1.68%). CONCLUSION: The method is of low cost and it is applicable for the determination of the low concentration of alprostadil for injection.
