Effect of Survivin antisense oligodeoxynucleotides on radiotherapy sensitivity in Hep-2 cells
- VernacularTitle:Survivin反义寡核苷酸对喉癌Hep-2细胞放疗的增敏作用
- Author:
Yongtao QU
;
Xiaoming LI
;
Xiuying LU
;
Wenrong DONG
- Publication Type:Journal Article
- Keywords:
Laryngeal Neoplasms;
Inhibitor of Apoptosis Proteins;
Oligonucleotides,Antisense;
Radiotherapy,Computer-Assisted;
Apoptosis
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the role of Survivin on radiotherapy inducing apoptosis and the effect of Survivin antisense oligodeoxynucleotides(ASODN)on the growth,apoptosis and radiotherapy sensitivity in Hep-2 cells.METHODS Survivin ASODN was synthesized and thansfected into hep-2 cells with lipofectin.The experiment consisted of three steps.①The efficiency of transfection was measured with flow cytometry technique.②The expression of survivin mRNA was observed with RT-PCR.③ Apoptosis and protein expression after radiotherapy was analyzed with flow cytometry.RESULTS ①Six hours after transfection of Survivin ASODN tagged with FITC into the cells,the efficiency of transfection was found to be 94% to 98% with flow cytometry.② Twenty four hours after transfection,Survivin mRNA was found decreased with the increasing of apoptosis,which presented a dose-dependent manner.③The apoptosis in the radiotherapy combined with Survivin ASODN transfected group was significantly higher than that in radiotherapy group only(P
