Platelet Activation During Hemodialysis Measured Through Expression of P-selectin.
- Author:
Hyung Jik KIM
1
;
Ja Ryong KOO
;
Dong Wan CHAE
;
Jung Woo NOH
Author Information
1. Department of Internal Medicine, College of Medicine, Hallym University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
P-selectin;
Hemodialysis;
Flow Cytometry;
ESRD;
Platelet
- MeSH:
Blood Platelets*;
Flow Cytometry;
Fluorescence;
Humans;
Kidney Failure, Chronic;
Macrophages;
Membranes;
Monocytes;
Mononuclear Phagocyte System;
P-Selectin*;
Platelet Activation*;
Renal Dialysis*;
Uremia
- From:Korean Journal of Nephrology
1998;17(3):461-465
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Recent studies suggested that platelet activation with surface expression of P-selectin may be related to a loss of viability and early clearing from circulation by monocytes and macrophages of the reticuloendothelial system. We have studied platelet activation during hemodialysis with a cuprophane membrane in eighteen patients with uremia. Blood samples were obtained at the begining of the hemodialysis(sample I) and after 1 hr of hemodialysis (sample II), and at the end of hemodialysis from the venous line (sample III). Platelet surface expression of P-selectin(CD62) was determined by flow cytometry. Percentage of positive platelets(% PP) of P- selectin was measured 43.9+/-15.6 % in sample I, 36.9+/-16.0% in sample II, and 40.1+/-13.1% in sample III. No statistical differences in P-selectin expression were observed in sample I, II, and III throughout the period of hemodialysis although platelet P-selectin expression after 1 hour of hemodialysis showed a slightly decrease(P=0.44). However, a significant increase in fluorescence occurred in samples from uremic patients(43.0+/-15.6%) with respect to low fluorescence was seen in normal control sera(14.6+/-11.2%). Our study shows that the substantial and irreversible platelet P-selectin expression might be contributing factors in early clearing of platelet from circulation in uremic patients.
