Effects of adiponectin against hydrogen peroxide induced myocardial injury in cultured rat neonatal cardiomyocytes
- VernacularTitle:脂联素对氧应激所致心肌损伤的影响
- Author:
Yunfei BIAN
;
Hui WANG
;
Chuanshi XIAO
- Publication Type:Journal Article
- Keywords:
Neonatal rat cardiomyocytes;
Adiponectin;
Cytarabine;
H2O2;
Signal transduction pathway
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effects of adiponectin on injury of the 3-4 d SD rat cardiomyocytes induced by the intervention of H2O2.METHODS:Primary cardiomyocytes were obtained from neonatal rat and were cultured by enzymatic digestion methods.The molecular marker was observed by ?-actin immunocytochemistry.Primary cultured 3-4 d cells were used in experiment,and the injury model was established by H2O2,and adiponectin and Ara-A were used for pre-treatment before cell culture.The morphological change of cardiomyocytes was observed under electron microscope.The contents of LDH,MDA and the activity of SOD were measured.The apoptosis of cardiomyocytes was detected by agarose gel electrophoresis and Annexin V/PI staining with flow cytometry.RESULTS:Adiponectin pretreatment significantly decreased the release of LDH(P
