Effect of fractalkine on the expression and secretion of MMP-2 in human monocytes
- VernacularTitle:Fractalkine对人单核细胞MMP-2的表达及分泌的影响
- Author:
Zheng LI
;
Zilin SUN
;
Li WANG
;
Ming FANG
- Publication Type:Journal Article
- Keywords:
Fractalkine;
Matrix metalloproteinase-2;
Monocytes;
Renal mesangial cells;
Diabetic nephropathies
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effect of fractalkine(CX3CL1,Fkn) on the expression and secretion of matrix metalloproteinase-2(MMP-2) in cultured human monocyte line U937 cells.METHODS:The cultured U937 cells were incubated with recombinant human Fkn,the supernatant of human renal mesangial cells(HRMC) and Fkn neutralizing antibodies for 24 h.The mRNA expression of MMP-2 was analyzed by RT-PCR.The production of MMP-2 in the supernatant was analyzed by gelatin zymography.RESULTS:The level of MMP-2 mRNA and protein in the cells incubated with recombinant human Fkn decreased compared to control group.Similarly,the level of MMP-2 mRNA in the cells incubated with the supernatant of HRMC reduced compared to control group.However,the level of MMP-2 mRNA and protein in the cells incubated with the supernatant of HRMC adding Fkn neutralizing antibodies increased compared to that incubated with the supernatant of HRMC.CONCLUSION:Fkn inhibits the expression and secretion of MMP-2 in cultured U937 cells.HRMC might mediate the expression and secretion of MMP-2 in U937 cells through Fkn.
