The effect of octreotide on growth inhibition of subcutaneous implanted tumor with human gallbladder carcinoma in nude mice
- VernacularTitle:奥曲肽对人胆囊癌裸鼠皮下种植瘤的抑制作用
- Author:
Jinghua WANG
;
Junmin WEI
;
Yalin LIU
;
Qing HE
;
Puxian TANG
;
Zhigang CHANG
- Publication Type:Journal Article
- Keywords:
Gallbladder Neoplasms;
Octreotide/ther use;
Cell Proliferation;
Apoptosis;
Nude Mice
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of octreotide on inhibition of growth of subcutaneously implantedtumor with human gall bladder cancer cells in nude mice,and to explore the mechanisms.Methods We established subcutaneous implanted tumor model in nude mice by using human gallbladder carcinomacell line GBC-SD.A total of 18 male nude mice bearing xenografts of the cell line were randomly divided into therapy and control groups,with 9 in each group.Octreotide was administered intraperitoneally at a dose of 100 ?g/(kg?d) to the therapy group and isovolumic normal saline was administered to the controlgroup for 6 weeks.All mice were put to death,and the weight and volume of the tumors were assayed.Flow cytometry was used to examine apoptosis of tumor cells.Immunohistochemical staining was used to examin the expression of p53,bcl-2,and Ki-67.Results The weight of implanted tumors in nude mice in the therapy group[(0.99?0.54)g] was lower than that in control group [(1.58?0.51)g,P
