In vitro killing effect of adenovirus-mediated herpes simplex virus thymidine kinase gene under regulation of hypoxic response element on hepatoma cell line HepG2
- VernacularTitle:低氧反应元件调控的腺病毒-胸苷激酶对肝癌细胞HepG2的体外杀伤作用
- Author:
Xiaozhong WANG
;
Qiquan PENG
;
Yunheng PENG
;
Wenpeng LIAO
- Publication Type:Journal Article
- Keywords:
Hypoxic response element;
Herpes simplex virus thymidine kinase;
Adenoviral vector;
Liver neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the in vitro killing effect of adenovirus-mediated herpes simplex virus thymidine kinase gene(HSV-TK) driven by hypoxic response element(HRE) on hepatoma cell line HepG2.METHODS:Recombinant adenoviral vector Ad-HRE-TK was constructed with HSV-TK under the control of HRE using AdEasy system.Then Ad-HRE-TK was transfected into hepatoma cell line HepG2 and the cells were cultured under normoxic or hypoxic conditions.After treated with GCV for 3 d,the sensitivity to GCV of HepG2 was measured by MTT method.RESULTS:Over 95% HepG2 cells infected with Ad-HRE-TK cultured under hypoxic condition were killed when the MOI was 100 and the concentration of GCV was 50 mg/L.On the contrary,no killing effect of GCV was observed in cells cultured under normoxic condition.CONCLUSION:HRE promotes the expression of HSV-TK specifically under hypoxic condition and induces the specific killing effect of GCV.
