Methylation and aberrant expression of Wnt antagonist SFRP2 in gastric cancer
- VernacularTitle:Wnt拮抗因子SFRP2在胃癌中的甲基化和异常表达
- Author:
Chenghai ZHAO
;
Xianmin BU
;
Ning ZHANG
;
Haipeng ZHANG
- Publication Type:Journal Article
- Keywords:
Wnt pathway;
Stomach neoplasms;
Methylation;
Frizzled-related protein-2
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine the methylation and expression of Wnt antagonist secreted Frizzled-related protein-2(SFRP2) in gastric cancer and to explore the role of SFRP2 in gastric carcinogenesis.METHODS:Methylation status of SFRP2 was detected by methylation-specific PCR(MSP).Real-time PCR was used to determine the expression of SFRP2 in gastric cancers and matched cancer adjacent normal tissues.SFRP2 expression in gastric cancer cell lines was determined by reverse-transcriptional PCR(RT-PCR) and Western blotting.RESULTS:SFRP2 methylation was found in 26(65%) gastric cancers and in 3(7.5%) matched cancer adjacent normal tissues.The frequency of methylation for SFRP2 was significantly higher in gastric cancers than that in matched normal tissues(x2=28.614,P
