Improvement of the Determination Method of Main Component in Lincomycin Hydrochloride Eye Drops
- VernacularTitle:盐酸林可霉素滴眼液中主药含量的测定方法改进
- Author:
Yan FANG
;
Bing SHU
;
Shantang ZHANG
;
Xiangqing CHEN
- Publication Type:Journal Article
- Keywords:
Lincomycin hydrochloride;
Eye drops;
HPLC;
Improvement
- From:
China Pharmacy
2005;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve the determination method for lincomycin hydrochloride eye drops so as to eliminate the interference of the bacterial inhibitor ethylparaben on the content of the main component.METHODS:The determination was performed by HPLC with phosphoric buffer (pH 6.8)-methanol (40∶ 60) as the mobile phase replacing the mobile phase of borax buffer (pH6.0)-methanol (4∶6) recommend in state chemicals standard technique (Method A).The contents of ethylparaben-contained sample and ethylparaben-free sample determined by HPLC were compared with those determined by Method A.RESULTS:There was no significant difference in the content of ethylparaben-free sample between the two determination methods,but the content of ethylparaben-contained sample determined by Method A was higher than both the labeled amount and that determined by the improved method.CONCLUSION:The improved method is applicable for the content determination of lincomycin hydrochloride eye drops.
