Mechanism of in Situ Intestinal Absorption of Mangiferin in Rats
- VernacularTitle:芒果苷大鼠在体肠道吸收机制研究
- Author:
Qin XU
- Publication Type:Journal Article
- Keywords:
Mangiferin;
In situ intestinal perfusion;
Absorption rate constants;
Percent absorption
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the mechanism of in situ intestinal absorption of mangiferin in rats.METHODS: The in situ intestinal perfusion model was employed.HPLC/UV was developed to determine the concentration of mangiferin in intestinal circulated fluid.The effects of mangiferin concentration,bile and absorption site on the absorption parameters were studied.RESULTS:The mangiferin at a concentration from 5.0 to 25.0 ?g?mL-1 had no influence on the intestinal absorption kinetics(Ka) but it did at a concentration below 12.5 ?g?mL-1 in biliary duct-ligated rats.The absorption rate constants(Ka) in descending order were 0.164 h-1(ileum),0.132 h-1(jejunum),0.125 h-1(colon) and 0.107 h-1(duodenum),respectively.CONCLUSION: The absorption of mangiferin is in line with first-order kinetics with passive diffusion absorption mechanism.Mangiferin is well absorbed at all segments of intestine in rats,and bile is conducive to the increase of the permeability coefficient of Mangiferin in intestine.
