Effects of n-butanol Extract of Morindae officinalis How on Apoptosis of Myocardial Cells in Neonatal Rats Undergoing Anoxia/Reoxygenation
- VernacularTitle:巴戟天正丁醇提取物对缺氧复氧乳鼠心肌细胞凋亡的影响研究
- Author:
Yan HE
- Publication Type:Journal Article
- Keywords:
Morindae officinalis HOW;
Anoxia-reoxygenation;
Myocardial cell;
Apoptosis
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the effect of n-butanol extract of Morindae officinalis How(BEM) on apoptosis of myocardial cells in neonatal rats undergoing anoxia/reoxygenation(A/R).METHODS: The rats were divided into 6 groups: normal group,A/R model,Danshen ingection(SM),BEM high,middle,low groups.The morphological changes of the apoptotic myocardial cells were observed using electron microscope.The apoptotic index was measured by in situ nick end labeling(TUNEL).The expression of Bcl-2 and the apoptotic protein Bax were detected by immunohistochemical staining.RESULTS: Morphocytology revealed the remarkable protective effect of BEM against apoptosis of myocardial cells during A/R.TUNEL test showed significantly lower apoptotic indexes in SM,BEM high,middle,low groups than in A/R group,and in all the SM,BEM high,middle,low groups,the positive expressed Bcl-2 gray scale value increased significantly(P
