Microemulsion Thin-layer Chromatography in the Separation and Identification of Various Effective Compositions in Changji'an Prescription
- VernacularTitle:微乳TLC法分离鉴定肠激安方中多种有效成分
- Author:
Hongmei TANG
- Publication Type:Journal Article
- Keywords:
Microemulsion;
TLC;
Changji'an prescription;
Separation;
Identification
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the microemulsion TLC for the separation and identification of various effective compositions in Changji'an prescription.METHODS: The compositions in Changji'an prescription were identified on polyamide film(fixed phase) with five distribution ratios of sodium lauryl sulphate-n-butanol-skellysolve C-H2O microemulsions as developing agents.The effects of factors such as the water content of the microemulsion and the temperature on the separation efficacy were studied.RESULTS: With microemulsion(75% water content) as developing agent,Rhizoma Coptidis in Changji'an prescription was detected;with microemulsion(75% water content)-formic acid(9.0∶1.0) as developing agent,Corydalis yanhusuo,Saposhnikovia divaricata,chlorogenic acid and Radix et Rhizoma Glycyrrhizae in Changji'an prescription were detected.CONCLUSION: Microemulsion TLC is simple,environmental-friendly,sensitive with ideal separation efficacy,and it can be used for the separation and identification of various compositions in Chinese patent medicines.
