Determination of Hesperidin in Yangweishu Tablets by RP-HPLC
- VernacularTitle:RP-HPLC法测定养胃舒片中橙皮苷的含量
- Author:
Zhanli CHEN
;
Aiping SUN
;
Runbo SHI
;
Zhiyao LI
;
Xuejun WEI
- Publication Type:Journal Article
- Keywords:
Yangweishu tablets;
Hesperidin;
Content determination;
RP-HPLC
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a RP-HPLC method for the determination of the content of hesperidin in Yangweishu tablets.METHODS: The samples were separated on octadecyl silane bonded gel silica column(150 mm?4.6 mm,5 ?m) with the mobile phase consisted of methanol-7% acetic acid solution(32∶68) under a detection wavelength of 283nm.RESULTS: The linear range of hesperidin was 49.6～892.8 ng(r=0.999 9) with an average recovery rate of 99.02%(RSD=0.45%,n=9).CONCLUSION:The method is simple,accurate,reproducible and applicable for the quality control of Yangweishu tablets.
