Determination of the dexamethasone in the cochlear tissue after postaurical and intramuscular injection
- VernacularTitle:大鼠耳后和肌肉注射地塞米松后内耳组织药物浓度分析
- Author:
Yunjuan LIN
;
Lisheng YU
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Ear,Inner;
Pharmacokinetics;
Enzyme-Linked Immunosorbent Assay;
Animal Experimentation
- From:Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To measure the dexamethasone concentration in the cochlear tissue after postaurical and intramuscular administration. METHODS After bilateral adrenalectomy,animals were divided into three groups:postaurical injection group,intramuscular injection group,and physiological saline injected blank control group.At 0.25,0.5, 2.5,5,12,24,72 h after injection(1mg/100g), cochleae together with the intact endolymphatic sac were removed and homogenated.After that, the dexamethasone was detected by enzymelinked immunosorbent assay(ELISA).RESULTS The dexamethasone levels in the cochlear tissue in postaurical injection group were significantly higher than that in the intramuscular injection group.In the postaurical administration group,a mean peak cochlear tissue concentration of(175.2?36.0) ng/ml was detected at 0.25 h after injection,then declined obviously 12 h and was below detection limits by 24h.While in the intramuscular administration group, a mean peak concentration was observed 2.5 h at the concentration of(141.1?8.9) ng/ml,then rapidly declined from 5h and beyond detection by 24 h.CONCLUSION Compared with intramuscular administration,the dexamethasone levels in the postaurical injection group were significantly higher, with peak concentration reached earlier,and last longer.
