The protective effect of CGRP on ET-1 induced injury of human hepatocyte
- VernacularTitle:CGRP对内皮素-1诱导肝细胞损伤的保护作用
- Author:
Shengdan NIE
;
Zhuori LI
;
Youde CAO
;
Pin LU
;
Yongzhong SHI
;
Shan LIANG
- Publication Type:Journal Article
- Keywords:
Hepatocytes;
Endothelin-1;
Calcitonin Gene-related Peptide
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of Calcitonin Gene-related Peptide(CGRP)on ET-1 mediated injury of human hepatocyte.Methods Human liver tissues obtained from patients undergoing partial hepatectomies were randomly divided into five groups:control group,liver perfused with D-Hank's solution group;liver perfused with ET-1 group and three liver perfased with ET plus CGRP(10-6M,10-7M,10-8M)treated groups.Collagenase digestion method was used to isolate human hepatocytes,then hepatocytes were cultured,and the level of MDA and TNF-?,the viability and proliferation of hepatocyte,and the hepatocyte function(ALT,Alb,Urea and LDH)were determined.Results As compared with control group,in ET-1 group,the viability and proliferation of hepatocytes,the level of Alb and Urea declined significantly(P
