Effects of different duration of sleep deprivation on neurogranin,PKC and CaMKⅡ mRNA expression in hippocampus and forebrain of rats
- VernacularTitle:不同睡眠剥夺时间对大鼠海马和前额皮层Ng、PKC、CaMKⅡ mRNA表达的影响
- Author:
Na ZHANG
;
Hongtao LIU
- Publication Type:Journal Article
- Keywords:
Sleep deprivation;
Neurogranin;
Protein kinase C;
Ca2+-calmodulin dependent protein kinase II
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effects of different duration of sleep deprivation (SD) on neurogranin (Ng),protein kinase C (PKC) and Ca2+-calmodulin dependent protein kinase II (CaMK II) mRNA expressions in hippocampus and forebrain of rats. METHODS:Male Wistar rats were divided into three groups:24 h,48 h and 72 h experimental groups. Each group was divided into sleep deprivation group (SD group) and control group (C group). SD model was established by sleep deprivation box. One step methods by Trizol was used to extract hippocampus and forebrain neuronal total RNA. The changes of Ng,PKC and CaMK II mRNA expressions were detected by SYBRA green I RT-PCR. RESULTS:(1) The expression of Ng mRNA in hippocampus of SD group was significantly lower than that in C group after 24 h,48 h and 72 h SD (P
