Intervention of gossypol and relationship between cognitive function and expressions of 11?-HSD1 and GR in brain of type 2 diabetic rats
- VernacularTitle:2型糖尿病大鼠脑内11?-HSD1和GR的表达与认知功能的关系及棉酚的干预作用
- Author:
Liang WU
;
Xiaoye WU
;
Huan WANG
;
Sanqiang NIU
;
Rongrong WANG
;
Xiaofei CHEN
;
Zhouxi FANG
;
Guorong CHEN
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Gossypol;
Hippocampus;
Receptors,glucocorticoid;
11 beta-hydroxysteroid dehydrogenase type 1
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effect of gossypol on the cognitive function of type 2 diabetic rats,and to explore its mechanism. METHODS:Thirty male Sprague-Dawley rats were divided into three groups randomly:normal group,type 2 diabetic group and gossypol treated group. After fed with high-fat diet for 4 weeks,the later two groups were injected with streptozotocin intraperitoneally to establish type 2 diabetic rat model. The animals in gossypol treated group were given gossypol at dosage of 15 mg/kg once per day for 4 weeks by gavage. Since 5th week,the times of gavages were changed into once per week at the same dosage and lasted to 12th week. Learning and memory abilities of rats were assayed with Morris water maze test. The concentration of blood glucose was measured by biochemical method. The levels of serum corticosterone and insulin were detected by enzyme-linked immunosorbent assay and radioimmunoassay,respectively. The protein expressions of 11?-HSD1 and GR in cerebral cortex and hippocampus were determined by Western blotting. The morphological changes of cerebral cortex and hippocampus were observed under light microscope and transmission electronic microscope,respectively. RESULTS:Compared to normal group,the karyopyknosis,dilation of golgiosome and mitochondria swelling of neuron from cerebral cortex and hippocampus were prominent in diabetic group. The concentrations of blood glucose,serum corticosterone and insulin increased significantly (P
