Expression of cathepsin B and cystatin C and its potential role in diabetic rats
- VernacularTitle:组织蛋白酶B和胱抑素C在糖尿病肾病大鼠肾组织中的表达及意义
- Author:
Huawei FANG
;
Yuqin FU
;
Yali HAN
;
Ye CHEN
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Cathepsin B;
Cystatin C
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the expressions of cathepsin B (CB) and cystatin C (CC) in different stage of diabetic rats and to investigates their potential roles.METHODS:Sixty rats were divided into diabetes mellitus group induced by intravenous injection of streptozotocin (55 mg/kg) and normal group injected with citrate buffer. Ten rats were sacrificed respectively at the end of fourth week,eighth week and sixteenth week in both groups. 24 h urine excretion was collected in rats before sacrifice. The blood and the kidney were also collected. The mRNA and protein expressions of CB and CC in kidney were detected by real time PCR and immunohistochemical staining,respectively.RESULTS:At the end of eighth week,the expression of Ccr,24 h urinary protein excretion,CB,CC in diabetic rats increased significantly,compared to the results at the fourth week (P
