Effects of microglia transplantation on treating cerebral infarction
- VernacularTitle:小神经胶质细胞移植对脑梗死的治疗作用
- Author:
Jian ZHANG
;
Min ZHANG
- Publication Type:Journal Article
- Keywords:
Brain infarction;
Microglia;
Blood-brain barrier;
Nerve growth factor;
Interleukin-10
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the regulating mechanism of microglia transplantation in treating cerebral infarction.METHODS:Focal cerebral infarction model was established by photochemistry. The histological and immunohistochemical methods were used to observe the effects of microglia transplantation on cerebral infarction. The expressions of nerve growth factor(NGF) and interleukin-10(IL-10) were detected by double fluorescent immunohistochemistry and Western blotting.RESULTS:Microglia marked with green fluorescence was observed in ischemic penumbra,indicating that microglia can be transplanted across the blood-brain barrier. Infarct volumes and death number of cells were significantly reduced compared to non-transplantation animals. The expressions of NGF and IL-10 were markedly increased in microglia in transplantation group.CONCLUSION:Microglia can be transplanted across the blood-brain barrier. The cells have protective effects in ischemic penumbra by secreting NGF and IL-10,which might serve as therapeutical method for treating cerebral infarction.
