Separation,Purification and Detection of Product of Bovine Hemoglobin Modified by Polyethylene Glycol
- VernacularTitle:聚乙二醇修饰牛血红蛋白产物的分离纯化和检测
- Author:
Jufeng SUN
;
Hongjuan LI
- Publication Type:Journal Article
- Keywords:
SC-mPEG;
Bovine hemoglobin;
Sodium dodecylsulfate-polyacrylamide gel electrophores;
Trinitrobenzenesulfonic acid
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: Researching the appropriate methods to separation,purification and detection the mixture of PEG modifier SC-mPEG with BHB.METHODS: Adopting cation-exchange chromatography and organic solvent precipitation to separate the mixture,then detecting it by SDS-PAGE and TNBS methods.RESULTS: Modifer and non-modifer product were separation by cation-exchange chromatography.The average modification rates of two peak product from cation-exchange chromatography are 52.95% and 14.19% detected by TNBS methods.CONCLUSIONS: Cation-exchange chromatography methods could be to separate and purify the SC-mPEG-BHB,TNBS methods coule be to detect the average modification rate of the SC-mPEG-BHB.
