Regulatory Effect of SNMC on Gene Expression of Smad Family in Pulmonary Fibrosis Model Rats
- VernacularTitle:复方甘草酸苷对肺纤维化模型大鼠Smad家族基因表达的调控作用研究
- Author:
Yan HE
;
Guozhong DENG
;
Tian GAO
- Publication Type:Journal Article
- Keywords:
SNMC;
Pulmonary fibrosis;
Model;
Rat;
Smad family;
Gene expression
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To observe the regulatory effect of SNMC on the gene expression of the Smad family in pulmonary fibrosis model rats.METHODS: A total of 174 rats were assigned to 6 groups: normal group,model group,positive prednisolone acetate group,SNMC(20,15,10 mL?kg-1?d-1) groups,with the latter 5 groups treated with bleomycin injection to induce pulmonary fibrosis model.At 24 h after modeling,the rats in 6 groups were treated with corresponding drugs day by day before being sacrificed at 1,12,28 days after first-time treatment for extracting of RNA from pulmonary tissues.The expression levels of Smad3,Smad4 and Smad7 were assayed by RT-PCR respectively.RESULTS: As compared with model group,the expression levels of Smad4 and Smad7 genes were down-regulated but the expression level of Smad3 gene was up-regulated in SNMC groups,whereas the expression levels of 3 kinds of genes were all down-regulated in positive prednisolone acetate group.CONCLUSION: SNMC is more effective than prednisolone acetate in reversing the abnormal expression of Smad family in pulmonary fibrosis model rats.
