Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium.
10.4142/jvs.2009.10.4.299
- Author:
So Yeong LEE
1
;
Kyong Ah YOON
;
Soo Hwa JANG
;
Erdene Ochir GANBOLD
;
Dembereldorj UURIINTUYA
;
Sang Mo SHIN
;
Pan Dong RYU
;
Sang Woo JOO
Author Information
1. Laboratory of Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
- Publication Type:Original Article ; Research Support, U.S. Gov't, Non-P.H.S.
- Keywords:
cancer cell screening;
cell monitoring;
infrared spectroscopy;
lung cancers;
phosphorylation
- MeSH:
*Carcinoma;
Cell Line, Tumor;
Epithelial Cells/*physiology;
Humans;
*Lung Neoplasms;
Respiratory Mucosa/*cytology;
*Spectrophotometry, Infrared
- From:Journal of Veterinary Science
2009;10(4):299-304
- CountryRepublic of Korea
- Language:English
-
Abstract:
The vibrational spectral differences of normal and lung cancer cells were studied for the development of effective cancer cell screening by means of attenuated total reflection infrared spectroscopy. The phosphate monoester symmetric stretching nus(PO3(2-)) band intensity at ~970 cm-1 and the phosphodiester symmetric stretching nus(PO2-) band intensity at ~1,085 cm-1 in nucleic acids and phospholipids appeared to be significantly strengthened in lung cancer cells with respect to the other vibrational bands compared to normal cells. This finding suggests that more extensive phosphorylation occur in cancer cells. These results demonstrate that lung cancer cells may be prescreened using infrared spectroscopy tools.
