Determination of the Related Substances in Aosaibos Crude Drug by HPLC
- VernacularTitle:HPLC法测定奥赛博司原料药中有关物质的含量
- Author:
Yujie CHEN
;
Peixun LIU
;
Ge HONG
;
Qi LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Aosaibos;
Related substances;
Content
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determine the contents of the related substances in Aosaibos crude drug by HPLC.METHODS: The determination was carried out on a SunFireTM RP C18 column with mobile phase consisted of phosphate buffer solution(pH 3.05)-acetonitrile(8∶2) at a flow rate of 1 mL?min-1.The detection wavelength was set at 220 nm;The sample size was 20 ?L and column temperature was set at room temperature.RESULTS: The main peak and the impurity peaks of 3 batchs of Aosaibos crude drug were well-separated.The contents of the related substances were all lower than 1.19%.CONCLUSION: The determination method is simple,sensitive and applicable for the content determination of the related substances of Aosaibos.
