Determination of Entrapment Efficiency of Gemcitabine Hydrochloride Liposome
- VernacularTitle:盐酸吉西他滨脂质体包封率的测定
- Author:
Ruizhi ZHANG
;
Yingjie DENG
- Publication Type:Journal Article
- Keywords:
Ultrafiltration;
Gemcitabine hydrochloride;
Liposome;
Entrapment efficiency;
HPLC
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for determining the entrapment efficiency of gemcitabine hydrochloride liposomes(GHL).METHODS: The liposome and the free gemcitabine hydrochloride were separated by ultrafiltration,and the content of free gemcitabine hydrochloride was determined by HPLC.The entrapment efficiency of gemcitabine hydrochloride liposomes was computed as well.RESULTS: The recovery rate in ultrafiltration methods was 97.8%～100.1% for blank substance versus 99.0%～100.1% for sample.The linear range of gemcitabine hydrochloride was 1.0～80.0 mg?L-1(r=0.999 3) and its average recovery rate was 98.7～101.2%,and its inter-day and intra-day RSD were all less than 3% and its average entrapment efficiency was 81.21%.CONCLUSION: The method is simple,accurate and applicable for the determination of the entrapment efficiency of gemcitabine hydrochloride liposomes.
