Determination of Chloramphenicol Content in Chloramphenicol Vaginal Soft Capsule by HPLC
- VernacularTitle:HPLC法测定氯霉素阴道软胶囊中氯霉素的含量
- Author:
Yan ZHOU
;
Yongyang SHAN
- Publication Type:Journal Article
- Keywords:
HPLC;
Chloramphenicol vaginal soft capsule;
Content
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for the determination of chloramphenicol content in chloramphenicol vaginal soft capsule.METHODS: The sample was separated on Kromasil C18 column with 0.1% sodium heptanesulfonate solution-acetonitrile(77∶23) as mobile phase at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 272 nm;The sample size was 10 ?L and the column temperature was maintained at 30 ℃.RESULTS: The linear range of chloramphenicol was 0.079～0.113 mg?mL-1(r=0.999 6) and its average recovery rate was 99.38%(RSD=0.42%).CONCLUSION: The method was proved to be specific,sensitive,accurate in results and suitable for the determination of chloramphenicol content in chloramphenicol vaginal soft capsule.
