Determination of Main Component in Ciprofloxacin Lactate Chitosan Nasal Drop by RP-HPLC
- VernacularTitle:RP-HPLC法测定环丙沙星壳聚糖滴鼻剂中主药的含量
- Author:
Huaixue JI
;
Yan WANG
;
Dongmei LV
;
Tao WANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Ciprofloxacin lactate;
Nasal drop;
Content
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determine the content of ciprofloxacin in ciprofloxacin lactate chitosan nasal drops by RP-HPLC.METHODS: The sample was separated on XTerra RP18 chromatographic column with 0.05 moL?L-1 citric acid-acetonitrile(80∶20) as mobile phase at a flow rate of 0.8 mL?min-1.The detective wavelength was set at 277 nm;The sensitivity was 2.000 AUFS;The column temperature was maintained at 30 ℃ and the sample size was 10 ?L.RESULTS: The linear ranges of ciprofloxacin lactate was 10～200 ?g?mL-1(r=0.999 9) and its average recovery rate was 99.46%(RSD=1.13%).CONCLUSION: This method is rapid,accurate and suitable for the determination of the content of ciprofloxacin in ciprofloxacin lactate chitosan nasal drops.
