Anti-apoptotic Effects of Cornus Officinals Iridoid Glycoside on Cellular Model of Alzheimer Disease Induced by Protein Phosphatase Inhibitor Okadaic Acid
- VernacularTitle:山茱萸环烯醚萜苷对冈田酸拟阿尔茨海默病细胞模型的抗凋亡作用研究
- Author:
Yanqi CHU
;
Lan ZHANG
;
Wen WANG
;
Wei LI
;
Li ZHANG
;
Lin LI
- Publication Type:Journal Article
- Keywords:
Cornel;
Iridoid glycoside;
Okadaic acid;
Apoptosis;
Alzheimer disease
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects and the mechanisms of cornel iridoid glycoside(CIG)on antiapoptosis in cellular model of alzheimer disease(AD)induced by the protein phosphatase inhibitor okadaic acid(OA).METHODS:The human neuroblastoma cell line SK-N-SH cells were preincubated with CIG for 24 h,and then incubated with OA 10 nmol for 6 h.The changes of apoptotic cells were observed by TUNEL method.Western blotting was used to determine the expression of apoptosis-regulating factors Bcl-2,Bcl-2 related X protein(Bax)and Caspase-3.RESULTS:The normal SK-N-SH cells spread well and no apoptotic cells appeared.In OA-treated model group,the number of apoptotic cells increased significantly,the expressions of Bax and Caspase-3 increased significantly,but Bcl-2 expression decreased significantly.In CIG(100 and 200 ?g?mL-1)-treated group,the number of apoptotic cells decreased significantly as compared with OA-treated model group,and the expressions of Bax and Caspase-3 decreased significantly whereas Bcl-2 expression increased significantly.CONCLUSION:CIG can inhibit the apoptosis of nerve cells through affecting apoptosis-regulating factors,suggesting its potential as a therapy for AD.
