Determination of Three Active Constituents in Coptidis Decoction for Detoxification by HPLC
- VernacularTitle:HPLC法同时测定黄连解毒汤中3种有效成分的含量
- Author:
Tao WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Baicalin;
Berberine hydrochloride;
Jasminoidin;
Content determination;
Coptidis decoction for detoxification
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of the contents of baicalin,berberine hydrochloride and jasminoidin in Coptidis decoction for detoxification.METHODS:The samples were separated on Shimazu Sim-pack VP-ODS C18(250 mm?4.6 mm,5 ?m)with mobile phase consisted of acetonitrile-(0.05% potassium dihydrogen phosphate+0.05% trolamine solution,with pH adjusted to 3.0 by phosphoric acid)in gradient elution under a detection wavelength of 254 nm.RESULTS:The linear ranges of jasminoidin,berberine hydrochloride and baicalin were 0.4～2.0 mg?mL-1(r=0.998 1),0.020～0.40 mg?mL-1(r=0.992 0)and 0.020～0.40 mg?mL-1(r=0.996 3),respectively,and their average recoveries were 99.8%,97.5% and 98.1%,respectively,with RSD at 1.8%,2.5% and 1.5%,respectively(n=6 for all the three).CONCLUSION:The established method is specific,sensitive,simple and applicable for the quality control of Coptidis decoction for detoxification.
