Determination of Galic Acid in Shiwei Xihuangcao Granules by HPLC
- VernacularTitle:HPLC法测定十味溪黄草颗粒中没食子酸的含量
- Author:
Hongwen HUANG
- Publication Type:Journal Article
- Keywords:
Shiwei xihuangcao granule;
Gallic acid;
HPLC;
Content determination
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of the content of gallic acid in Shiwei xihuangcao granules.METHODS:The sample was separated on DiamonsilTM C18(200 mm?4.6 mm,5 ?m)with 0.1% phosphoric acid solution(containing 0.1% triethylamine)-methanol(95∶5)as mobile phase under a detection wavelength of 220 nm.RESULTS:The linear range of gallic acid was 0.1～0.5 ?g(r=0.999 6)and its average recovery was 98.63%(RSD=0.4%,n=6).CONCLUSION:This method was proved to be simple,accurate,reproducible,and suitable for quality control of Sh-iwei xihuangcao granules.
