Determination of Imperatorin in Qingwen Jiedu Tablets by RP-HPLC
- VernacularTitle:RP-HPLC法测定清瘟解毒片中欧前胡素的含量
- Author:
Jizhu LIU
;
Fuhai WU
;
Hui LIU
;
Yan LU
- Publication Type:Journal Article
- Keywords:
Qingwen jiedu tablets;
Imperatorin;
RP-HPLC;
Content determination
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of imperatorin in Qingwen jiedu tablets(heat-clearing and detoxifying agent).METHODS:The chromatographic separation was performed on Diamonsil-C18(250 mm?4.6 mm,5 ?m)with column temperature kept at 35 ℃.The mobile phase consisted of methanol-water(70∶30)at a flow rate of 0.8 mL?min-1 with detection wavelength set at 300 nm.RESULTS:The linear range of imperatorin was 2.7～27.0 ?g?mL-1(r=0.999 9)with its average recovery at 98.9%(RSD=1.44%,n=6).CONCLUSION:The established method was proved to be well-separated,sensitive,accurate and applicable for the quality control of Qingwen jiedu tablets.
