Effects of Different Concentration of Methanol on 5 Anthraquinone Constituents in Semen Cassiae
- VernacularTitle:不同浓度甲醇对决明子中5种蒽醌类成分含量的影响考察
- Author:
Xiaobin GUAN
- Publication Type:Journal Article
- Keywords:
Methanol;
Concentration;
Semen Cassiae;
HPLC;
Anthraquinone constituents
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of different concentration of methanol on 5 anthraquinone constituents in Semen Cassiae.METHODS:Semen Cassiae was extracted with different concentration of methanol(95%,80% and 30%).The contents of aloe-emodin,rhein,emodin,chrysophanol and physcione in Semen Cassiae were determined by HPLC.RESULTS:The contents of aloe-emodin,rhein,emodin,chrysophanol and physcione were 0.001 0 ～0.018 1 mg?g-1,0.010 4～0.063 7 mg?g-1,0.020 9～0.253 5 mg?g-1,0.003 1～0.915 7 mg?g-1 and 0.005 1～0.126 3 mg?g-1,respectively,The average recoveries were 96.34%～97.16% and the RSDs of all were less than 3%(n=6).CONCLUSION:There were great differences in the contents of the 5 anthraquinone constituents in Semen Cassiae extracted with different concentration of methanol,and a best extraction efficacy was achieved for Semen Cassiae extracted with 95% methanol while the worst extraction efficacy was noted if extracted with 30% methanol.
