Determination of Plasma Concentration of Paclitaxel in Tumor Patients by RP-HPLC
- VernacularTitle:RP-HPLC法测定肿瘤患者血浆中紫杉醇浓度
- Author:
Yuan BIAN
;
Yi LIU
;
Yun YE
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Paclitaxel;
Blood drug concentration
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method to determinate the plasma concentration of paclitaxel in tumor patients.METHODS:Paclitaxel was extracted from plasma with organic phase(ethyl-acetate)by two-step extraction on Tianhe Kromasil C18 column(250 mm?4.6 mm,5 ?m)with a mobile phase consisted of acetonitrile-methanol-water(40∶25∶40)at a flow rate of 1 mL?min-1.The detective wavelength was set at 227 nm and the column temperature was maintained at 35 ℃.RESULTS:The linear range of paclitaxel was 0.05～5.00 mg?L-1(r=0.999 7)with average recovery rate at 98.75%～100.44%.Both intra-day RSD and inter-day RSD were less than 5%(n=5).The plasma concentration-time profile in 11 patients after iv infusion of paclitaxel was in line with a two-compartment model.CONCLUSION:This established method is simple,accurate,reproducible and applicable for clinical determination of blood drug concentration and pharmacokinetic studies.
