Effects of Le Er Mai on apoptosis of hippocampus neuronal cells in anaphase of cerebral ischemic reperfusion injury in rats
- VernacularTitle:乐尔脉对脑缺血再灌注损伤后期大鼠海马神经细胞凋亡的作用
- Author:
Junqin WANG
;
Shengchun WANG
;
Jianfeng LI
;
Zengfa CAO
- Publication Type:Journal Article
- Keywords:
Le Er Mai;
Brain ischemia;
Reperfusion;
Apoptosis;
Fas(Aop-1/CD95)
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects and mechanism of Le Er Mai(LEM) on the apoptosis of hippocampus neuronal cells in the anaphase of cerebral ischemic reperfusion injury in rats.METHODS:A rat model of middle cerebral artery occlusion reperfusion(MCAO) was produced with the intraluminal filament.During reperfusion for 30 d after 2 h of ischemia,the TUNEL staining methods were used to detect apoptosis of hippocampus neuronal cells,and immunohistochemical technique were employed to examine the protein expression of Fas,Bax,caspase-3 and caspase-9 in the hippocampial.The gene expressions of fas,bax,caspase-3 and caspase-9 in hippocampial were examined by RT-PCR.RESULTS:After 2 h ischemia and 30 d reperfusion,compared with sham-operated group,TUNEL-positive staining cells and expression levels of Fas,Bax as well as caspase-3 and caspase-9 obviously increased,and the mRNA expressions of fas,bax,caspase-3 and caspase-9 in hippocampial markedly up-regulated in model group.Compared with model group,LEM at dose of 2.00 g/kg or 0.87 g/kg,and flunarizinum significantly reduced apoptosis and decreased the protein expressions of Fas,Bax,caspase-3 and caspase-9 in hippocampial,and down-regulated the mRNA expressions of fas,bax,caspase-3 and caspase-9(P
