The Influence of Sodium Salicylate on the Expression of Na~+-K~+-2Cl~-
- VernacularTitle:水杨酸钠对大鼠耳蜗NKCC_1 mRNA表达的影响
- Author:
Keqing YAO
;
Zhiwu HUANG
;
Kun YANG
;
Juan HUANG
- Publication Type:Journal Article
- Keywords:
NKCC1 co-transporter;
Sodium salicylate;
Outer hair cell electromotility;
Fluorescence quantitative PCR
- From:
Journal of Audiology and Speech Pathology
1998;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the different expression levels of Na+-K+-2Cl-co-transporter NKCC1 mRNA in the cochlea of rats after sodium salicylate injection and to explore the mechanism underlying the change of outer hair cells,induced by different salicylate administration.Methods Twenty-four normal adult rats were randomly divided into four groups with six rats in each group. Rats in control group,did not recieve sodium salicylate injection. The other three groups were acute group,chronic group,and recovered group according to the different doses of sodium salicylate.The fluorescence quantitative PCR method was used to detect the expression levels of NKCC1 mRNA in the rat cochleas of the four groups.Results NKCC1 mRNA was expressed in all of the four groups.After sodium salicylate injection,the expressions of NKCC1 mRNA in chronic and recovered group were higher than that in control group(P
