Diltiazem Inhibit Differention and Maturation of Dendritic Cells in Peripheral Blood by Enzymatically Modified LDL
- VernacularTitle:地尔硫抑制酶修饰低密度脂蛋白对外周血中树突状细胞分化成熟的影响研究
- Author:
Wei HE
;
Aihua CHEN
;
Xudong SONG
- Publication Type:Journal Article
- Keywords:
Enzymatically modified low density lipoprotein;
Dendritic Cell;
Diltiazem;
Expression
- From:
China Pharmacy
2005;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the effect of enzymatically modified low density lipoprotein(E-LDL) on differation and maturation of human dendritic cells(DC) in peripheral blood and the effect of diltiazem on it.METHODS: Human monocytes were isolated by using gradient centrifugation method and cultured in DC Cellgro medium containing rhGM-CSF and rhIL-4 for 5 days,then DC was then obtained.DC were treated with E-LDL or E-LDL plus diltiazem for 48 hours,the morphological changes were observed under the microscope,and the FACS was used to investigate the expression of DC(CD1a,CD80,CD86 and HLA-DR).PBS group was set up for comparison.RESULTS: The morphological changes were nor observed.The expressions of CD1a,CD80,CD86 and HLA-DR in each groups were significant different(P
