Purification Technology of Camellia Saponin Using D101 Macroporous Adsorption Resin
- VernacularTitle:D101型大孔吸附树脂纯化油茶皂苷的工艺研究
- Author:
Hongbing LI
;
Ling TANG
;
Baomin FENG
;
Yongqi WANG
- Publication Type:Journal Article
- Keywords:
D101 macroporous adsorptive resin;
Camellia saponin;
Content;
Adsorption quantity;
loading volume of sample
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study thc purification technology of camellia saponin using D101 macroporous adsorption resin.METHODS:The loading volume of sample and the adsorption quantity of D101 macroporous adsorption resin and the concentration and volume of alcohol in eluting water -soluble foreign substances and total cemallia saponins were investigated taking the content of total cemallia saponins as index.RESULTS:The loading volume of sample of D101 macroporous adsorption resin was 3 fold that of resin D101(or 3 BV,resin bed volume) and its adsorption quantity was 566 mg?g~(-1)(crude drug);3 BV distilled water and 1 BV 20%ethanol were used for the elution of the water-soluble foreign substances,and 3BV of 85%ethanol was used for the elution of total cemallia saponins.CONCLUSION:D101 macroporous resin has good purification efficacy for camellia saponins.The purification process is simple with low cost,and it is applicable for large - scale industrialized production.
