Determination of Hydrochlorothiazide in New Wenshen Xiaozhong Pill by HPLC
- VernacularTitle:HPLC法测定新温肾消肿丸中氢氯噻嗪的含量
- Author:
Yi YUAN
- Publication Type:Journal Article
- Keywords:
New wenshen xiaozhong pill;
HPLC;
Hydrochlorothiazide;
Content determination
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an HPLC method for the determination of hydrochlorothiazide in New wenshen xiaozhong pill.METHODS:The determination was performed on a Kromasil 60- 5CN(250 mm?4.6 mm,5?m).The mobile phase consisted of methanol- 0.05 mol?L~(-1) potassium dihydrogen phosphate(pH = 3.0 adjusted by phosphoric)(49:51). The UV detection wavelength was set at 226 nm.RESULTS:The linear range of hydrochlorothiazide was 2.227～44.540?g?mL~1(r =0.999 9) with an average recovery rate of 100.70%(RSD = 1.64%,n = 6).CONCLUSION:The method is simple,rapid,accurate and applicable for the quality control of New wenshen xiaozhong pill.
