Up-regulation of the tumor-suppressing gene expression in nasopharyngeal carcinoma cells induced by Arsenic Trioxide
- VernacularTitle:亚砷酸诱导人低分化鼻咽癌细胞系肿瘤抑制基因表达
- Author:
Haihui LIANG
;
Yuefei ZHANG
;
Jun YAO
- Publication Type:Journal Article
- Keywords:
Arsenites;
Nasopharyngeal Neoplasms;
Genes,Tumor Suppressor
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE This study is to investigate the effects on nasopharyngeal carcinoma cell lines(CNE-2Z)induced by As2O3 and its possible mechanisms.METHODS CNE-2Z cells were treated with various concentrations of As2O3 for different times.The IC50 values were detected by trypan blue stain assay to choose the optimal concentration of As2O3.Cell cycle redistribution was analyzed by flow cytometry.The expression levels of p16、 RASSF1A、DNMT 3B mRNA were assessed by RT-PCR.RESULTS The suppression of cell proliferation by As2O3 was time and dose-dependent.After being treated with different concentration of As2O3,the IC50 values of As2O3 were(1.50?0.05),(1.09?0.13),(0.65?0.04)?mol/L at 24,48,and 72h,respectively.As2O3 also arrested CNE-2Z cells in G2/M phase of cell cycle.After CNE-2Z cells being treated with As2O3,the expression of RASSF1A mRNA were up-regulated(P
