Relationship between synaptophysin expression in rats hippocampus CA3 region and chronic intermittent hypoxia
- VernacularTitle:大鼠海马CA3区突触素与慢性间歇性缺氧
- Author:
Bing LI
;
Feng ZHANG
;
Guohua HU
- Publication Type:Journal Article
- Keywords:
Rats;
Animal Experimentation;
Anoxia;
Hippocampus;
Synapses
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the effect of chronic intermittent hypoxia and removal of hypoxia on learning and memory ability in Sprague-Dawley rats and the synaptophysin expression in hippocampus CA3 region.METHODS Experiments were performed on 24 three-month-old male Sprague-Dawley rats,They were randomly assigned to three experimental groups(n=8/group):unhandled control group(UC),chronic intermittent hypoxia group(CIH),and Removal of hypoxia group(RH).Rats in UC group were raised normally,those in CIH group suffered 8H intermittent hypoxia everyday,the duration of experiment was 4 weeks,and those in RH group was treated as same as those in CIH group in the first 4 weeks and were raised normally in the next 4 weeks.The learning and memory ability of rats in each group were assessed with the Morris water maze test at the end of the experiments.The expression of synaptophysin in hippocampus CA3 region was investigated by immunohistochemistry staining and image analysis.RESULTS ①Morris water maze test learning scores(place navigation):From the beginning,the escape latency in CIH rats was significantly longer than that in UC rats and RH rats(P
