Determination of Plasma Concentration of Flurbiprofen by RP- HPLC
- VernacularTitle:RP-HPLC法测定人血浆中氟比洛芬的浓度
- Author:
Chunyan ZHANG
;
Jian GU
- Publication Type:Journal Article
- Keywords:
RP HPLC;
Flurbiprofen;
Plasma concentration;
Pharmacokinetics
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBECTIVE:To establish a RP -HPLC method for the determination of plasma concentration of flurbiprofen. METHODS:By external standard method,the plasma sample after precipitation with acetonitrile was determined on Symmetry shield C_(18) chromatographic column with the mobile phase consisted of buffer phosphate(pH 7.0) - acetonitrile(75:25) at a flow rate of 1.0 mL?min~(-1).The detection wavelength was set at 247 nm.RESULTS:The linear range of flurbiprofen was 0.05～20?g?mL~(-1)(r=0.999 1) with the detection limit of flurbiprofen at 0.05?g?mL~(-1).The average recoveries at low,middle and high concentrations all ranged between 96.1%and 107.1%,and both the intra - day RSD and the inter-day RSD were less than 10%,meeting the standard required of the methodology.CONCLUSION:The method developed in this study was proved to be simple,accurate,rapid,and suitable for the monitoring of concentration and pharmacokinetic study of flurbiprofen.
