Impact of galectin-1 expression on LoVo cell apoptosis
- VernacularTitle:Galectin-1表达对LoVo细胞凋亡的影响
- Author:
Huasheng TONG
;
Yali ZHANG
;
Bo JIANG
;
Lei SU
- Publication Type:Journal Article
- Keywords:
Colorectal Neoplasms;
galectin-1;
Gene Reconstruction;
Apoptosis
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of LoVo cell growth by galectin-1.Methods Eukaryotic expression vector of galectin-1 was constructed and transfected into LoVo cells using lipofectamineRM 2000.Immunochemistry was employed to detect galectin-1 expresson.LoVo cell proliferation and apoptosis were observed.Results Galectin-1 eukaryotic expression vector pEGFP-C1/GAL1 was successfully constructed.Three cell clones,p-GAL1-LoVo and p-LoVo,transfected with pEGFP-C1/GAL1 and pEGFP-C1 correspondingly,and LoVo were cultured successfully.Galectin-1 expression downregulated Bcl-2 level only occurring in p-GAL1-LoVo cells.p-GAL1-LoVo cell proliferation was similar to p-LoVo and LoVo cells,but p-GAL1-LoVo cell apoptosis was increased(9.61?0.56)%,as compared with p-LoVo and LoVo cells,[(3.56?0.53)% and(3.46?0.46)% respectively](P
