Effect of recombinant MIF on lung fibroblast proliferation and collagen synthesis in vitro
- VernacularTitle:巨噬细胞移动抑制因子促进肺成纤维细胞增殖和胶原合成
- Author:
Peifen CHEN
;
Yaling LUO
;
Wenyan LAI
;
Xiaowen XING
;
Siming HU
- Publication Type:Journal Article
- Keywords:
Macrophage migration-inhibitory factors;
Fibroblasts;
Collagen;
Asthma
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the influence and mechanism of recombinant macrophage migration inhibitory factor(rMIF) on fibroblasts.METHODS: MRC-5 fibroblasts were divided into two groups: the treated group was treated with rMIF(25-100 ?g/L,12 h,24 h or 48 h) and the control was non-rMIF treatment.The activity of proliferation in both groups was investigated and compared by CCK-8 means.Synthesis of collagen in the culture supernatants was detected by the hydroxyproline.The expression of collagen type I mRNA was examined using RT-PCR analysis.The level of collagen type I protein induced by rMIF was quantified by Western blotting.RESULTS: The production of proliferation ratio of fibroblasts treated with 50 ?g/L and 100 ?g/L rMIF at 24 h or 48 h were increased obviously(P
