Significance and change of SCF/KIT following cerebral ischemia reperfusion
- VernacularTitle:脑缺血再灌注后干细胞因子和KIT受体的变化及意义
- Author:
Yun LUO
;
Wenjing ZHU
;
Yun XU
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Neurons;
Stem cell factor;
Receptors,tyrosine kinase
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the expressions and significance of stem cell factor(SCF) and tyrosine kinase receptor(KIT) in neurons after cerebral ischemia reperfusion injury.METHODS: Western blotting was used to examine the expression of SCF and KIT in the cortex after cerebral ischemia and oxygen glucose deprivation(OGD) neurons,then the OGD neurons preincubated with SCF and SCF-Ab were assessed the cell viability by MTT to determine its significance.RESULTS: ①Compared to control group,the expressions of M-SCF and S-SCF in cortex increased markedly while KIT decreased significantly in ipsilateral hemisphere.However,no obvious change in the contraleteral hemisphere was observed.②Compared to control group,the levels of S-SCF and M-SCF were increased significantly in the OGD neurons while KIT was not obvious changed.③The cell viability of OGD neurons decreased significantly.Preincubation with SCF inhibited cell damage while SCF-Ab deteriorated OGD injury.CONCLUSION: The expression of SCF may be induced by cerebral ischemia reperfusion,which can protect the neurons from ischemia injury.
