Ovarian serous cystadenocarcinoma cell apoptosis induced by inhibition of Bax inhibitor-1(bi-1) gene expression with RNA interference
- VernacularTitle:RNA干扰阻抑Bax i nhi bitor-1(bi-1)基因表达对卵巢癌细胞凋亡的影响
- Author:
Meihong ZHANG
;
Keyuan ZHOU
- Publication Type:Journal Article
- Keywords:
Bax inhibitor-1;
RNA interference;
Ovarian neoplasms;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the inhibition of the Bax inhibitor-1(bi-1) gene expression induced with RNA interference in HO8910PM and HO8910 cell lines in addition to the apoptotic induction.METHODS: After transfection of recombinant plasmid which expresses bi-1shRNA into HO8910PM and HO8910 cells,bi-1 mRNA and protein levels were detected by reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting.Apoptotic HO8910PM and HO8910 cells were detected by Hoechst 33258/PI fluorescent staining and flow cytometry.RESULTS: Compared with the control group,the expressions of bi-1 gene at mRNA and protein levels were declined evidently in the cells transfected with four candidate siRNA plasmids(P
