Factors Influencing the Preparation and Quality of Bovine Serum Albumin Liposomes Coated with Ntrimethyl Chitosan
- VernacularTitle:N-三甲基壳聚糖包覆牛血清白蛋白脂质体的制备及质量影响因素研究
- Author:
Jinna CAO
;
Yingjie DENG
;
Jukui SUN
;
Xiaofei GAO
;
Wenxiu LI
;
Wei WEI
- Publication Type:Journal Article
- Keywords:
N-trimethyl chitosan;
Bovine serum albumin;
Liposomes;
Entrapment efficiency;
Influencing factors
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare bovine serum albumin(BSA)liposomes coated with N-trimethyl chitosan(TMC) and study the factors influencing the quality of the preparation.METHODS:BSA liposomes were prepared by the method of reverse-phase evaporation.TMC polymers for coating liposomes were synthesized by quaternary amination reaction between chitosan and methyl iodide.The TMC-coated BSA liposomes were prepared.The entrapment efficiency of BSA liposomes was determined by high speed centrifugation-Coomassie brilliant blue G-250 dye method.The effects of the composition ratio of soybean lecithin(PC):cholesterol(CH):phospbatidylserine(PS),the mass ratio of TMC to total lipid and the ionic strength on particle size and entrapment efficiency were investigated.RESULTS:All the factors investigated had influences on particle size and entrapment efficiency.The optimal formulation was as follows:PC:CH:PS was 8:9:1,TMC to lipid phase ratio was 0.25:1 and ionic strength was less than 20 mmol?L~(-1).The entrapment efficiency prepared in the above conditions was (46.82?2.07)%.CONCLUSION:The TMC-coated BSA liposomes were obtained successfully and the preparation had uniform particle size and good stability.
