Determination of Ethambutol Hydrochloride in Ethambutol Hydrochloride-Pyrazinamide-Rifampicin-Isoniazid TabletsⅡ
- VernacularTitle:HPLC法测定乙胺吡嗪利福异烟片Ⅱ中盐酸乙胺丁醇的含量
- Author:
Dahai PANG
;
Yanyan LI
;
Wenzhong HAN
;
Zhuo WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Ethambutol hydrochloride;
Ethambutol hydrochloride-pyrazinamide-rifampicin-isoniazid tabletsⅡ;
Content determination
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of the content of ethambutol hydrochloride in ethambutol hydrochloride-pyrazinamide-rifampicin-isoniazid tabletsⅡ.METHODS:The sample was separated on Alltima CN column.The mobile phase was acetonitrile-triethylamine(50:50)water solution at a flow rate of 1.0 mL?min~(-1).The diluent agent was Na_2HPO_4 solution.The detective wavelength was set at 200 nm;the column temperature was maintained at room temperature and the sample size was 20?L.RESULTS:The linear range of ethambutol hydrochloride was 100.2～601.2?g?mL~(-1)(r=0.999 98)and its average recovery was 99.8%(RSD=0.374%).CONCLUSIONS:The method is simple,accurate and reproducible,and it can be used for the content determination of ethambutol hydrochloride in this tablet.
